Fig. 6.

IGET induced tumor regression depended on adaptive immune response and not due to a direct effect of plasmid DNA. Nude mice (a and b) and TLR9−/− mice (c and d) were intradermally injected with 0.5 × 10⁶ B16F10 cells. When the tumor reached a volume of 7 to 15 mm3, 2x25μg of pIL-12 were injected intradermally in the healthy skin directly surrounding the tumor. Electrotransfection was realized using HV-MV electrical parameters. Two days later, pIRE parameters were applied on previously treated tumors. As control, mice were only treated with pIL-12 GET, pIRE or untreated. Tumor volume (a, c) and mice survival (b, d) was followed until tumor volume reached 1200 mm³. *p < 0.05, ***P < 0.001 (Log-rank (Mantel-Cox) Test between untreated control, pIL-12 GET, pIRE and IGET treated tumor. The number of mice is indicated on the graph in 2 independent experiments