Table 1.

The oligonucleotide primers and probes for real-time PCR used in this study

Organism	Target gene	Primer sequence (5′-3′)	Probe (5′-3′)	Citation
S. pneumoniae	lytA	F: ACGCAATCTAGCAGATGAAGC R: TGTTTGGTTGGTTATTCGTGC	FAM-TTTGCCGAAAACGCTTGATACAGGG-BHQ	[29]
H. influenzae	bexA	F: GGCGAAATGGTGCTGGTAA R: GGCCAAGAGATACTCATAGAACGTT	HEX-CACCACTCATCAAACGAATGAGCGTGG- BHQ	[29]
GBS	cfb	F: AGCTCTATTAGAAGTACATGCT R: CATTTGCTGGGCTTGATTATT	ROX-ATCAAGTGACAACTCCACAAGTGGTAA- BHQ2	[30]
E. coli	uid	F: GTGTGATATCTACCCGCTTCGC R: GAGAACGGTTTGTGGTTAATCAGGA	ROX-TCGGCATCCGGTCAGTGGCAGT-BHQ2	[31]
N. meningitidis	ctrA	F: GCTGCGGTAGGTGGTTCAA R: TTGTCGCGGATTTGCAACTA	FAM- CATTGCCACGTGTCAGCTGCACAT-BHQ	[32]
L. monocytogenes	iap	F: CTAAAGCGGGAATCTCCCTT R: CCATTGTCTTGCGCGTTAAT	HEX- CTTCTGGCGCACAATACGCTAGCACT- BHQ	[33]
mumps virus	Fusion protein	F:TCTCACCCATAGCAGGGAGTTATAT R: GTTAGACTTCGACAGTTTGCAACAA	HEX-AGGCGATTTGTAGCACTGGATGGAACA- BHQ	[34]
herpes simplex virus	polymerase	F:CATCACCGACCCGGAGAGGGAC R: GGGCCAGGCGCTTGTTGGTGTA	FAM- CCGCCGAACTGAGCAGACACCCGCGC- BHQ	[35]
Japanese encephalitis virus	polyprotein	F: AGAACGGAAGAYAACCATGACTAAA R: CCGCGTTTCAGCATATTGAT	ROX-ACCAGGAGGGCCCGG- BHQ2	[36]
enterovirus	polyprotein	F: CCGGCCCCTGAATGC R: CACCGGATGGCCAATCCA	ROX-AACCGACTACTTTGGGTGTCCGTGTTTC- BHQ2	[37]
adenovirus	hexon	F: GCCACGGTGGGGTTTCTAAACTT R: GCCCCAGTGGTCTTACATGCACATC	CY5-TGCACCAGACCCGGGCTCAGGTACTCCGA -BHQ2	[38]
varicella zoster virus	DNA polymerase	F: CGGCATGGCCCGTCTAT R: TCGCGTGCTGCGGC	HEX-ATTCAGCAATGGAAACACACGACGCC- BHQ1	[39]