Fig. 3.

Effect of Cd on the xylem protein profile as revealed by label-free shotgun analyses. At two weeks of age, the hydroponically grown Brassica napus seedlings were exposed to 0 and 10 μM of Cd for three days. a Volcano scatter plot showing the identified and quantified proteins in xylem sap. Proteins unaffected by Cd are depicted in black, and proteins changing as a result of Cd (ANOVA, p ≤ 0.05) are depicted in red (left represents decreasing, right represents increasing). b Cluster of proteins controlled by Cd (ANOVA p < 0.05). Heatmaps report high (red) and low level (blue). CK-1, CK-2, CK-3, Cd-1, Cd-2, and Cd-3 represent biological replicates xylem sap samples from control and Cd-treated Brassica napus plants respectively