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. 2019 Jun 26;33:2058738419859699. doi: 10.1177/2058738419859699

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© The Author(s) 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — PD-L1 was a target gene of miR-200a-3p and could be indirectly up-regulated by MALAT1: (a) miR-200a-3p binding sequence of PD-L1 3’-UTR indicated that PD-L1 was a potential target of miR-200a-3p. (b) qRT-PCR showed that transfection of miR-200a-3p mimics significantly attenuated the mRNA levels of endogenous PD-L1 in both A549 and CAL-12T cells. (c) Western blot showed that transfection of miR-200a-3p mimics significantly attenuated the protein levels of endogenous PD-L1 in both A549 and CAL-12T cells. (d) miR-200a-3p significantly repressed the luciferase activity of wide type 3’-UTR of PD-L1, but did not change the luciferase activity of mutated 3’-UTR of PD-L1 in A549 cells. (e) Western blot showed that overexpression of MALAT1 significantly increased the protein levels of endogenous PD-L1 in both A549 and CAL-12T cells.

**P < 0.01. ***P < 0.001.