Figure 7.

Biohybrid nanoparticles favor the efficacy of the anti-CTLA-4 therapy by reshaping the tumor microenvironment. Individual tumor samples were collected at the end point for each mouse, and the tumor-infiltrating cells were analyzed. (A) The percentage of CD8⁺ TILs was normalized against the tumor volumes of each mouse to account for the size of tumor masses. (B) Infiltration of myeloid cells (defined as CD11b⁺ cells) into the tumor microenvironment. (C) DCs were defined as CD11c⁺ and CD11b⁺ double positive cells within the tumor microenvironment. Data were normalized against the tumor. (D) Total percentages of activated and mature DCs, defined as either CD86⁺ (gray), CD80⁺ (black) single positive, or CD86⁺ CD80⁺ double positive cells (red). (E) Relative distribution and polarization of the DCs subsets for each group. All graphs represent the mean ± SEM. Statistical analysis done with unpaired Student’s t test; *p < 0.05 and **p < 0.01.