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. 2019 Feb 5;132(3):319–328. doi: 10.1097/CM9.0000000000000066

Figure 4.

Figure 4

The 70% ethanol extract of male flowers of E. ulmoides inhibited the activation of the nuclear factor-κB pathway in lipopolysaccharide-stimulated RAW 264.7 cells. (A) Representative expression for NF-κB p65, pIκBα and pIKKα/β detected by Western blotting. (B) Densitometric quantification of NF-κB p65 with GAPDH as loading control. (C) Densitometric quantification of pIκBα with GAPDH as loading control. (D) Densitometric quantification of pIKKα/β with GAPDH as loading control. 1. Negative control, 2. LPS, 3. LPS+EF 30 μg/mL, 4. LPS+EF 20 μg/mL, 5. LPS+EF 10 μg/mL. ∗P < 0.05 vs. negative control; †P < 0.05 vs. LPS only treatment (n = 3). LPS only treatment: Cells were treated with 1 μg/mL LPS in Dulbecco modified Eagle's medium containing 10% fetal bovine serum. EF:70% ethanol extract of male flowers of E. ulmoides; TGs: Tripterygium glycosides; NF-κB: Nuclear factor-κB; IκB: Inhibitor of kappa B; IKK: Inhibited IκB kinase; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; LPS: Lipopolysaccharide.