Skip to main content
letter
. 2019 Apr 5;132(7):868–872. doi: 10.1097/CM9.0000000000000157

Figure 1.

Figure 1

High concentration of glucose up-regulated α-SMA expression and triggered ERS in NRK-52E cells. Cells were cultured in medium with different level glucose (NC group: 5.6 mmol/L; H1 group: 15 mmol/L; H2 group: 25 mmol/L; H3 group: 50 mmol/L), or medium containing 25 mmol/L glucose in different time for 24 h (H/24 h group) or 48 h (H/48 h group). The results showed that high glucose induced the overexpression of α-SMA protein (A). The expression of GRP78 protein and phosphorylation of PERK and eIF2α were increased in high glucose medium, while the express of PERK and eIF2α protein did not change (B and C). ∗P < 0.05 vs. NC group, †P < 0.05 vs. H1 group and H/24 h group. α-SMA:α-smooth actin; ERS: Endoplasmic reticulum stress; eIF2α: Eukaryotic translation-initiation factor 2α; GRP78: 78kd-glucose-reglulated protein; Mann: Mannitol; NC: Normal control; PERK: Protein kinase R-like ER kinase.