Fig. 3.

—Sequence comparisons among the ACCD of Sciadopitys, gnetophytes, and other gymnosperm representatives. (a) Identification of predicted nr-ACCD proteins from Sciadopitys and three genera of gnetophytes. The putative nr-ACCDs were aligned with the plastid (pt) ACCDs of three other gymnosperms: Ginkgo, Cycas, and Pinus. Orange shading denotes the TP sequences as predicted by LOCALIZER and TargetP. Red shading denotes the TPs predicted by TargetP only. The gaps in the Gnetum parvifolium transcriptome assembly are highlighted with blue shading. (b) ML tree of ACCD sequences. The nr-ACCD sequences of Sciadopitys and gnetophytes (bold) were aligned with the plastid ACCD sequences of other 53 sampled gymnosperm species. Bootstrapping supports for the node are shown when they are >50%. A black arrow indicates a plastid-to-nuclear accD gene transfer event. Marchantia, Sphagnum, and Anthoceros were designated as the outgroups.