Table 1.

STIM1 knockdown disrupts EB3 comet-like dash dynamics^a

	STIM1-CTRL	STIM1-KD
Average dash number per frameb in growth cone	0.031 ± 0.0131	0.018 ± 0.0130
Average dash velocityc in growth cone	0.17 ± 0.07	0.11 ± 0.04*
Average distanced per dash (μm) in growth cone	1.07 ± 0.36	0.71 ± 0.26**
Average track length (μm) in growth cone	9 ± 5.85	3.81 ± 1.58***
Average filopodial dash velocityc	0.37 ± 0.13	0.29 ± 0.05****

^aMicrotubule dynamics were examined by overexpressing EB3-YFP in STIM1-CTRL and STIM1-KD growth cones. Dash velocity, distance, and track length in whole growth cone were analyzed in 6 dashes of STIM1-CTRL (54 in 9 separate growth cones) and STIM1-KD (36 in 6 separate growth cones) growth cones. Filopodial EB-dash velocity was analyzed in single filopodia from STIM1-CTRL (12) and STIM1-KD (13) growth cones expressing EB3-YFP.

^bImages acquired every 6 s. Number of dashes per frame were normalized to growth cone area.

^cValues are μm/s ± SD.

^dValue calculated from average of distance traveled by a single dash per frame.

*p = 0.000006;

**p = 0.0000015;

***p = 0.0000013;

****p = 0.036.