Figure 4.

No synaptic activity defects and pathology developed in αSyn KO neurons. A, B, Synaptic activity remained unchanged after αSyn PFFs treatment for 4 and 7 d in αSyn KO neurons. C, No αSyn pathology detected by ICC staining in αSyn KO neurons. Syn211 antibody was used to label the human αSyn PFFs seeds added to the cultures. 81a: p-αSyn, Syn211: human αSyn PFFs seeds, MAP2: dendrites. Electrophysiology data collected from three independent batches of cultures, ICC staining data collected from two independent batches of cultures. Scale bar, 50 μm.