Skip to main content
. 2018 Jun 27;38(26):5872–5887. doi: 10.1523/JNEUROSCI.3321-17.2018

Figure 5.

Figure 5.

Lrfn2 mRNA is detected in both glutamatergic and GABAergic neurons. Expression of SALM1/Lrfn2 mRNA in glutamatergic and GABAergic neurons was determined by FISH. Coronal sections from mouse brains (male, 8 weeks) were triply stained for SALM1/Lrfn2 and Vglut1/2 (glutamatergic neuron markers) and Gad1/2 (GABAergic neuron markers) and counterstained with the nuclear dye DAPI. A mixture of two probes (Vglut1 + Vglut2 or Gad1 + Gad2) was used to label all glutamatergic or GABAergic neurons. The indicated cortical and hippocampal regions in the image at left were enlarged on the right to highlight the expression of Lrfn2 mRNA in both glutamatergic and GABAergic neurons; Lrfn2 expression in GABAergic neurons is further highlighted by arrows. Scale bar, 50 μm.