Figure 4.

IF studies of distribution of MECR and lipoylated proteins in control and KO mice aged between 3 and 6 months. Anti-MECR and anti-LA panels are from hybridizations of consecutive sections, so the images do not co-register perfectly. Antisera with reasonable specificity against either target were only available from the same species source animal, thus not allowing for dual staining of the identical material. A Hoechst 33342 stain was used to label cellular nuclei. A dataset including these images was used for our quantitation of MECR and lipoylation intensities shown in Table 2. Scale bar, 40 μm.