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. 2018 Jun 6;38(23):5313–5324. doi: 10.1523/JNEUROSCI.3097-17.2018

Figure 3.

Figure 3.

The C2 domains are essential for stimulus-evoked neurotransmitter release. Electric stimulus–evoked EPSCs were recorded from the body wall muscle of the indicated genotypes or transgenes. A, Representative traces of evoked EPSCs. B, C, Quantifications of the mean amplitude and charge transfer of evoked EPSCs. The synaptotagmin isoforms that possibly mediate the remaining evoked release in snt-1 mutants are shown in Figure 3-1. ###p < 0.001 compared to wild type; **p < 0.01, ***p < 0.001 compared to SNT-1C2AB rescue. n.s., Nonsignificant compared to SNT-1C2AB rescue. Significance tests were performed using one-way ANOVA followed by Dunnett's test. D, E, Representative traces and quantification graph of sucrose-evoked responses from wild-type and snt-1 mutants. The recording conditions were the same as those for evoked EPSCs. The total released quanta were analyzed during the 5s sucrose puff. The number of worms analyzed for each genotype is indicated in the bar graphs. ***p < 0.001 compared to wild type. The significance test was performed using Student's t test. Data are means ± SEM. The number of worms analyzed for each genotype is indicated in the bar graphs.