Table 3.
Statistical comparison of the Ca2+ responses to a series of IPIs in each GAL4 strain or condition
| Neuronal type/condition | Data | Statistical method | Df | Sum Sq | Mean Sq | F-value | Pairwise | p-value | Significance | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Figure 1C | JO-B | Integrated Ca2+ response | One-way ANOVA with repeated measures | IPI | 9 | 1.59 | 0.177 | 0.758 | — | 0.655 | |
| Individual | 7 | 10.4 | 1.48 | 6.36 | — | 1.15E-05 | *** | ||||
| Residuals | 63 | 14.7 | 0.233 | ||||||||
| Figure 1D | AMMC-B1 | Integrated Ca2+ response | One-way ANOVA with repeated-measures post hoc Bonferroni multiple comparisons | IPI | 9 | 17.0 | 1.89 | 6.14 | 2.24E-06 | *** | |
| Individual | 8 | 52.5 | 6.57 | 21.4 | 3.11E-16 | *** | |||||
| Residuals | 72 | 22.1 | 0.307 | ||||||||
| 15–25 | 5.32E-05 | *** | |||||||||
| 15–35 | 3.71E-04 | *** | |||||||||
| 15–45 | 3.22E-04 | *** | |||||||||
| 15–55 | 5.23E-04 | *** | |||||||||
| 15–65 | 2.23E-05 | *** | |||||||||
| 15–75 | 2.40E-04 | *** | |||||||||
| 15–85 | 5.11E-06 | *** | |||||||||
| 15–95 | 2.01E-06 | *** | |||||||||
| 15–105 | 9.47E-06 | *** | |||||||||
| Other pairs | 1.00 | ||||||||||
| Figure 2C | AMMC-B1 (Rdl-RNAi) | Integrated Ca2+ response | One-way ANOVA with repeated-measures post hoc Bonferroni multiple comparisons | IPI | 9 | 19.2 | 2.14 | 21.7 | <2E-16 | *** | |
| Individual | 10 | 58.9 | 5.89 | 59.8 | <2E-16 | *** | |||||
| Residuals | 90 | 8.9 | 0.0990 | ||||||||
| 15–25 | 1.70E-19 | *** | |||||||||
| 15–35 | 3.00E-09 | *** | |||||||||
| 15–45 | 7.09E-05 | *** | |||||||||
| 15–55 | 4.18E-04 | *** | |||||||||
| 15–65 | 0.0659 | ||||||||||
| 15–75 | 1.96E-04 | *** | |||||||||
| 15–85 | 0.0623 | ||||||||||
| 15–95 | 0.106 | ||||||||||
| 15–105 | 0.100 | ||||||||||
| 25–35 | 1.39E-04 | *** | |||||||||
| 25–45 | 6.46E-09 | *** | |||||||||
| 25–55 | 8.16E-10 | *** | |||||||||
| 25–65 | 9.49E-13 | *** | |||||||||
| 25–75 | 2.01E-09 | *** | |||||||||
| 25–85 | 1.03E-12 | *** | |||||||||
| 25–95 | 4.54E-13 | *** | |||||||||
| 25–105 | 4.96E-13 | *** | |||||||||
| 35–55 | 0.365 | ||||||||||
| 35–65 | 3.71E-03 | ** | |||||||||
| 35–75 | 0.614 | ||||||||||
| 35–85 | 3.97E-03 | ** | |||||||||
| 35–95 | 2.10E-03 | ** | |||||||||
| 35–105 | 2.26E-03 | ** | |||||||||
| Other pairs | 1.00 | ||||||||||
| (Control) | The scores are the same as in Figure 1D | ||||||||||
| Figure 4A | AMMC-LN | Integrated Ca2+ response | One-way ANOVA with repeated measures | IPI | 9 | 3.33 | 0.370 | 1.54 | — | 0.165 | |
| Individual | 5 | 21.0 | 4.20 | 17.4 | — | 1.41E-09 | *** | ||||
| Residuals | 45 | 10.8 | 0.241 | ||||||||
| Figure 4B | AMMC-B2 | Integrated Ca2+ response | One-way ANOVA with repeated measures | IPI | 9 | 0.117 | 0.0130 | 0.499 | — | 0.867 | |
| Individual | 5 | 0.454 | 0.0908 | 3.48 | — | 9.67E-03 | ** | ||||
| Residuals | 45 | 1.18 | 0.0261 | ||||||||
| Figure 5B | AMMC-B1 (VT022100 > TNT) | Integrated Ca2+ response | One-way ANOVA with repeated measures | IPI | 9 | 0.408 | 0.0453 | 1.76 | — | 0.0879 | |
| Individual | 10 | 26.9 | 2.69 | 104 | — | <2E-16 | *** | ||||
| Residuals | 90 | 2.32 | 0.0258 | ||||||||
| AMMC-B1 (VT022100 > IMPTNT) | Integrated Ca2+ response | One-way ANOVA with repeated-measures post hoc Bonferroni multiple comparisons | IPI | 9 | 2.20 | 0.244 | 4.99 | — | 9.52E-06 | *** | |
| Individual | 14 | 50.8 | 3.63 | 74.2 | — | <2E-16 | *** | ||||
| Residuals | 126 | 6.16 | 0.0490 | ||||||||
| 15–25 | 9.85E-03 | ** | |||||||||
| 15–35 | 3.45E-03 | ** | |||||||||
| 15–45 | 1.69E-04 | *** | |||||||||
| 15–55 | 1.25E-05 | *** | |||||||||
| 15–65 | 6.91E-04 | *** | |||||||||
| 15–75 | 3.81E-03 | ** | |||||||||
| 15–85 | 2.24E-05 | *** | |||||||||
| 15–95 | 5.74E-05 | *** | |||||||||
| 15–105 | 8.72E-05 | *** | |||||||||
| Other pairs | 1.00 | ||||||||||
| Figure 5C | AMMC-B1 (R55C02 > TNT) | Integrated Ca2+ response | One-way ANOVA with repeated measures | IPI | 9 | 0.696 | 0.0773 | 1.44 | — | 0.178 | |
| Individual | 13 | 17.2 | 1.32 | 24.7 | — | <2E-16 | *** | ||||
| Residuals | 117 | 6.27 | 0.0536 | ||||||||
| AMMC-B1 (R55C02 > IMPTNT) | Integrated Ca2+ response | One-way ANOVA with repeated-measures post hoc Bonferroni multiple comparisons | IPI | 9 | 4.26 | 0.473 | 3.95 | — | 2.28E-04 | *** | |
| Individual | 12 | 39.2 | 3.27 | 27.3 | — | <2E-16 | *** | ||||
| Residuals | 108 | 12.9 | 0.120 | ||||||||
| 15–25 | 0.0487 | * | |||||||||
| 15–35 | 1.48E-03 | ** | |||||||||
| 15–45 | 8.02E-04 | *** | |||||||||
| 15–55 | 1.03E-02 | * | |||||||||
| 15–65 | 2.26E-02 | * | |||||||||
| 15–75 | 1.84E-03 | ** | |||||||||
| 15–85 | 2.45E-04 | *** | |||||||||
| 15–95 | 2.65E-03 | ** | |||||||||
| 15–105 | 2.41E-04 | *** | |||||||||
| Other pairs | 1.00 | ||||||||||
| Figure 5D | AMMC-B1 (R26C02 > TNT) | Integrated Ca2+ response | One-way ANOVA with repeated measures | IPI | 9 | 0.340 | 0.0380 | 1.26 | — | 0.266 | |
| Individual | 12 | 44.7 | 3.73 | 125 | — | <2E-16 | *** | ||||
| Residuals | 108 | 3.22 | 0.0300 | ||||||||
| AMMC-B1 (R26C02 > IMPTNT) | Integrated Ca2+ response | One-way ANOVA with repeated-measures post hoc Bonferroni multiple comparisons | IPI | 9 | 1.79 | 0.198 | 3.09 | — | 0.00248 | ** | |
| Individual | 12 | 13.13 | 1.09 | 17.0 | — | <2E-16 | *** | ||||
| Residuals | 108 | 6.95 | 0.0643 | ||||||||
| 15–25 | 1.38E-03 | ** | |||||||||
| 15–35 | 2.68E-02 | * | |||||||||
| 15–45 | 2.42E-02 | * | |||||||||
| 15–55 | 2.74E-02 | * | |||||||||
| 15–65 | 2.95E-02 | * | |||||||||
| 15–75 | 4.22E-02 | * | |||||||||
| 15–85 | 3.49E-02 | * | |||||||||
| 15–95 | 0.317 | ||||||||||
| 15–105 | 3.16E-04 | *** | |||||||||
| Other pairs | 1.00 | ||||||||||
Asterisks indicate statistical significance: ***p < 0.001, **p < 0.01, *p < 0.05. For a statistical comparison of the Ca2+ responses to a series of IPIs in each condition of AMMC-B1 neurons labeled by the R45D07-LexA driver, see Table 3-1.
Df, Degree of freedom; Sq, square.