Bootstrap analysis confirms that neuronal IL ensembles activated by cue-induced reinstatement of ethanol (EtOH) and saccharin (Sac) seeking, detected by FISH against different cFos mRNA species are highly nonrandom, independent of the order of cues presented for reinstatement. A, B, Histograms of random distributions of cells expressing one, both, or none of the cFos mRNA obtained by 100,000 shuffles. Vertical and dashed lines indicate the fractions of cells experimentally determined. C, Quantification of cells expressing one, both, or none of the cFos mRNA species, according to the reward reinstated first.