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. 2018 Jan 10;38(2):278–290. doi: 10.1523/JNEUROSCI.1649-17.2017

Figure 4.

Figure 4.

Phosphorylation of CSPα at Ser10 and Ser34 promotes the interaction with HSP70 in PC12 cells. A, Coimmunoprecipitation assay with anti-Myc Ab was performed with COS7 transfected with Myc-tagged HSP70 and FLAG-tagged WT CSPα in the presence or absence of 1 μm TPA. The interaction of Myc-tagged HSP70 and FLAG-tagged WT CSPα was normalized to the level of the control, as shown in the bar graph. The results are expressed as mean ± SEM (n = 4, *p < 0.05, unpaired t test). B, Coimmunoprecipitation assay with anti-FLAG Ab was performed with COS7, transfected with Myc-tagged HSP70, and FLAG-tagged WT CSPα in the presence or absence of 1 μm TPA. The interaction of Myc-tagged HSP70 and FLAG-tagged WT CSPα was normalized to the level of the control, as shown in the bar graph. The results are expressed as mean ± SEM (n = 9, *p < 0.05, unpaired t test). C, Coimmunoprecipitation assay with anti-FLAG Ab was performed with COS7, transfected with Myc-tagged HSP70, and FLAG-tagged HSP40 in the presence or absence of 1 μm TPA. The interaction of Myc-tagged HSP70 and FLAG-tagged HSP40 was normalized to the level of the control, as shown in the bar graph. The results are expressed as mean ± SEM (n = 4, p > 0.05, unpaired t test). D, Coimmunoprecipitation assay with anti-FLAG Ab was performed with COS7, transfected with Myc-tagged HSC70, and FLAG-tagged HSP40 in the presence or absence of 1 μm TPA. The interaction of Myc-tagged HSC70 and FLAG-tagged HSP40 was normalized to the level of the control, as shown in the bar graph. The results are expressed as mean ± SEM (n = 4, p > 0.05, unpaired t test). E, Coimmunoprecipitation assay with anti-FLAG Ab was performed with COS7, transfected with Myc-tagged HSP70, and FLAG-tagged CSPα (WT and Ser/Ala mutants). The interaction of Myc-tagged HSP70 and FLAG-tagged CSPα (WT and Ser/Ala mutants) was normalized to the level of WT. The results are expressed as mean ± SEM (n = 5, *p < 0.05 vs WT, 1-way ANOVA with post hoc Dunnett's test). F, Coimmunoprecipitation assay with anti-FLAG Ab was performed with COS7, transfected with Myc-tagged HSP70, and FLAG-tagged CSPα (WT, Ser/Asp, and Ser/Glu mutants). The interaction of Myc-tagged HSP70 and FLAG-tagged CSPα (WT, Ser/Asp, and Ser/Glu mutants) was normalized to the level of WT, as shown in the bar graph. The results are expressed as mean ± SEM (n = 3, *p < 0.05 vs WT, 1-way ANOVA with post hoc Dunnett's test).