Exo70 is localized at synaptic boutons at the NMJ. Confocal micrographs of larval NMJs on muscles 6 and 7 of segment A3, colabeled with anti-HRP and anti-Exo70Maguro (A–I) or anti-HRP and anti-Exo70Sake (J–L). Low-magnification confocal micrographs showing the entire NMJ of (A) w1118 and (B) exo70KG08051/Df(3L)BSC815. Scale bar, 2.5 μm. High-magnification confocal micrographs of (C) w1118, (D) exo70KG08051/Df(3L)BSC815, (E) exo70KG08051/MB04553, (F) C380>UAS-exo70, (G, J) C380,C57 double driver control, (H, K) C380,C57>exo70GD12140-dsRNA, and (I, L) C380,C57>exo70KK101154-dsRNA. Scale bar, 2.5 μm. All experiments were performed three times independently.