Depletion of PNNs alters GABAergic transmission onto large DCN neurons. A, Representative traces of eIPSCs in large DCN neurons with (right) and without (left) incubation of ChABC (0.25 U/ml). B, ChABC treatment increased the amplitude and reduced the PPR of eIPSCs significantly. *p < 0.05, **p < 0.01 using unpaired Student's t test. C, No effect on rise (left) or decay (right) kinetics of eIPSCs. D, Representative averaged eIPSCs induced by 10 stimuli at 100 Hz in large DCN neurons. E, Mean amplitudes of phasic eIPSCs normalized to the first phasic IPSC in each condition. ChABC treatment enhanced the depression of eIPSCs (p < 0.0001 using regression analysis). Ctrl, Control.