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. 2018 Jul 4;38(27):6130–6144. doi: 10.1523/JNEUROSCI.3238-17.2018

Figure 7.

Figure 7.

Effects of PNN depletion on rebound firing in large DCN neurons. A, Representative membrane potential and firing before and after repetitive stimulation of GABAergic synapses (100 Hz for 100 ms) in large DCN neurons in control (left) and ChABC-treated neurons (right). Constant holding currents were injected into neurons to keep the prestimulus firing rate similar at 10–20 Hz. B, No difference in the resting membrane potential with the constant holding current between the control (open bar, n = 7) and ChABC-treated neurons (red bar, n = 5). The membrane potential was corrected for a 15 mV junction potential. ChABC treatment increased the mean peak amplitude of IPSP responses during the repetitive stimulation. **p < 0.01 using unpaired Student's t test. C, The effects of ChABC treatment on the mean frequency after the repetitive stimulation. D, The mean increases of rebound firing in large DCN neurons in control (n = 7) and ChABC-treated slices (n = 5) are shown as percentages to the baseline firing rate in each condition. *p < 0.05 using unpaired Student's t test. E, Representative membrane potentials and action potential firings before and after the injection of hyperpolarizing current (400 pA, 500 ms) in the control (left) and ChABC-treated (right) large DCN neurons. Constant holding currents were injected with large DCN neurons to keep the prestimulus firing rate at between 10 and 20 Hz. F, No difference in the resting membrane potential with the constant holding current between the control (open bar, n = 13) and ChABC-treated (red bar, n = 7) neurons. The membrane potential was corrected for a 15 mV junction potential. ChABC treatment had no effect on the amplitude of sag induced by the injection of hyperpolarizing current. G, Changes in the firing rates after the hyperpolarizing current injection in the control neurons (n = 13) and ChABC-treated large DCN neurons (n = 7). H, There is no difference in the percentage increase of rebound firing between the control and ChABC-treated large DCN neurons. Ctrl, Control.