Figure 3.

Resistance of spliced 5-HT1A 3′-UTR to miR135. SKN-SH or HEK-293 cells were transfected with luciferase constructs: vector (pGL3P), WT, unspliceable (U, Unspliced), or constitutively spliced (LS, Spliced) human 5-HT1A 3′-UTR, treated with control miR (Ctrl) or miR135, and analyzed for relative luciferase activity normalized to pGL3P (100%). Data are shown as mean ± SEM of three experiments. *p < 0.05, ***p < 0.001 versus Ctrl or as shown; ##p < 0.01, ###p < 0.001 vs WT; one-way ANOVA, Tukey's post hoc test.