Figure 4.

Normal LTP induction in GluA1 C811S mutant mice. A, E/I balances in CA1 pyramidal neurons. Representative traces of compound PSP in WT (left) and CS/CS (middle) mice are shown. The EPSP/IPSP ratios were calculated and plotted (right) (WT: n = 14 slices from 3 mice; CS/CS: n = 17 slices from 3 mice. B, Hippocampal CA3-CA1 LTP was induced using 4 trains of high-frequency stimulation (each train includes 100 Hz for 1 s, the intertrain interval is 10 s). fEPSP slopes (normalized to percentage of baseline) were plotted over time (left). Representative traces of fEPSPs before and after LTP induction are also shown. Scale bars, 0.5 mV, 10 ms. The summary plot of LTP (averaged for the last 10 min) level is shown in the right (WT: n = 11 slices from 5 mice, CS/CS: n = 11 slices from 5 mice). Error bars indicate SEM. C, Hippocampal CA3-CA1 LTD was induced by the bath application of 20 μm NMDA for 3 min. The normalized fEPSP slopes were plotted over time (left). Representative traces of fEPSP before and after chem-LTD induction are also shown. Scale bars, 0.5 mV, 10 ms. The summary plot of chem-LTD (averaged for last 10 min) level is shown on the right (WT: n = 11 slices from 5 mice, CS/CS: n = 11 slices from 5 mice). Error bars indicate SEM.