Figure 8.
VMP GABAergic neurons exhibited higher c-Fos expression during night. A, Example of double-labeled cells (indicated by arrows) for c-Fos (green) and mCherry (GABAergic cells; red) counterstained with DAPI (nuclei; blue) in VGAT-CreM4/VMP mice. Scale bar, 10 μm. B, Line drawings showing typical distribution of c-Fos/mCherry double-labeled cells (red circles). C, Wakefulness during 3 h before killing the mice. D, Percentage of double-positive neurons for c-Fos and mCherry among mCherry-positive neurons in the VMP. **p < 0.01 compared with light period. Data are presented as the mean ± SEM (n = 8). RN, Red nucleus; SN, substantia nigra; IP, interpeduncular nucleus.