Table 3.

PCR protocol for D1^Cre/+ and A2A^Cre/+

Step	Initiation/Melting	Denaturation	Annealing	Elongation	Amplification	Hold
Temperature (°C)	94	94	65–55	72	72	4
Duration (min)	5	0.25	0.5	0.17	5	∞
Cycles	1	40 (1–10: ΔTAnneal − 1°C/cycle)			1