Table 4.
PCR protocol for D1tdTomato/+
| Step | Initiation/melting | Denaturation | Annealing | Elongation | Amplification | Hold |
|---|---|---|---|---|---|---|
| Temperature (°C) | 95 | 95 | 60 | 72 | 72 | 4 |
| Duration (min) | 3 | 0.25 | 0.25 | 0.25 | 1 | ∞ |
| Cycles | 1 | 35 | 1 | |||
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