Table 4.
Step | Initiation/melting | Denaturation | Annealing | Elongation | Amplification | Hold |
---|---|---|---|---|---|---|
Temperature (°C) | 95 | 95 | 60 | 72 | 72 | 4 |
Duration (min) | 3 | 0.25 | 0.25 | 0.25 | 1 | ∞ |
Cycles | 1 | 35 | 1 |
Step | Initiation/melting | Denaturation | Annealing | Elongation | Amplification | Hold |
---|---|---|---|---|---|---|
Temperature (°C) | 95 | 95 | 60 | 72 | 72 | 4 |
Duration (min) | 3 | 0.25 | 0.25 | 0.25 | 1 | ∞ |
Cycles | 1 | 35 | 1 |