Table 5.

PCR protocol for Ai9

Step	Initiation/melting	Denaturation	Annealing	Elongation	Amplification	Hold
Temperature (°C)	94	94	65–60	72	72	10
Duration (min)	2	0.33	0.25	0.17	2	∞
Cycles	1	38 (1–10: ΔTAnneal − 0.5°C/cycle)			1