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. 2018 Jan 24;38(4):858–877. doi: 10.1523/JNEUROSCI.0843-17.2017

Figure 3.

Figure 3.

Identification of an early upregulated gene set implicated in cell division using RNA sequencing. A, Volcano plot representing the fold change (FC) and false discovery rate-adjusted p value of all 19,459 considered genes in an RNA sequencing analysis conducted on P30 control (Ctr; n = 5) and Miz1ΔPOZ (n = 3) sciatic nerves, highlighting twofold (false discovery rate, <10−6) regulated genes in red (up) or blue (down). B, List of almost twofold deregulated genes that also have a highly significant false discovery rate of <10−6. C–D, Gene-expression analysis relative to Gapdh, validating a set of deregulated genes (dark red in B) by qRT-PCR analysis at P30 (C) or P90 (D) in Ctr and Miz1ΔPOZ sciatic nerves; n = 4 animals per genotype and time point. E, GO analysis of the deregulated gene list depicted in B representing all biological processes with p < 10−5. C and D, Asterisks indicate the results of a two-tailed, unpaired Student's t-test (*p < 0.05, **p < 0.01, ***p < 0.001).