Figure 6.

Sustained proliferation of Miz1ΔPOZ Schwann cells causes augmented nerve diameters. A, B, Longitudinal sections of sciatic nerves dissected at indicated ages were stained for the Schwann cell marker Sox10 and counterstained with Hoechst. Quantification of all (Hoechst⁺) nuclei per mm² (A) or of Sox10⁺ Schwann cells per mm² (B). n = 3–9 animals per genotype and time point. C, TUNEL assay was performed on longitudinal sections of sciatic nerves dissected from P60 control (Ctr) and Miz1ΔPOZ animals using sections of an adult thymus as a positive control. Sections were counterstained with Hoechst and the percentage of TUNEL⁺ nuclei among all nuclei was determined. n = 4 animals per genotype, counting 3400–5600 cells per animal. D, E, Representative macroscopic pictures of P30 or P200 Ctr or Miz1ΔPOZ sciatic nerves (D) and the corresponding quantification of the mean nerve diameter (E). The diameter was determined at three different positions throughout each individual nerve of a sciatic-nerve pair. n = 3–5 animals of each genotype and time point. A, B, C and E Asterisks indicate the results of a two-tailed, unpaired Student's t-test (*p < 0.05, **p < 0.01, ***p < 0.001; ns = not significant; p > 0.05).