Table 1.
List of antibodies used
| Antigens | Clonality | Host | Catalog # | Company | Immunohistochemistry | Western blotting | ChIP |
|---|---|---|---|---|---|---|---|
| GAPDH | Mono | Mouse | 5G4-6C5 | HyTest | — | 1:2000 | — |
| Histon H3 | Poly | Rabbit | ab1791 | Abcam | — | 1:2000 | — |
| H3K4me3 | Mono | Rabbit | 04-745 | Millipore | — | 1:2000 | — |
| H3K9me3 | Poly | Rabbit | ab8898 | Abcam | — | 1:2000 | — |
| H3K36me2 | Poly | Rabbit | C15310127 | Diagenode | 1:250* | 1:2000 | 2 μg |
| JUN | Mono | Rabbit | 9165 | Cell Signal Technology | — | 1:500 | — |
| Ki67 | Mono | Rat | M7249 | Dako | 1:200* | — | — |
| Miz1 | Mono | Mouse | a | — | — | 1:400 | — |
| Miz1 | Poly | Rabbit | sc-22837 | Santa Cruz Biotechnology | — | — | 3 μg |
| MPZ | Poly | Rabbit | ab31851 | Abcam | — | 1:1000 | — |
| Myc-TAG | Poly | Rabbit | 2272 | Cell Signal Technology | — | 1:1000 | — |
| SOX10 | Poly | Rabbit | SI058R06 | DCSb | undiluted* | — | — |
| α-mouse HRP | Poly | Goat | 172-1011 | Bio-Rad | — | 1:7500 | — |
| α-rabbit HRP | Poly | Goat | 172-1019 | Bio-Rad | — | 1:7500 | — |
| α-rabbit A488 | Poly | Goat | A-11008 | Invitrogen | 1:400 | — | — |
| α-rabbit A546 | Poly | Goat | A-11010 | Invitrogen | 1:400 | — | — |
| α-rat A633 | Poly | Goat | A-21094 | Invitrogen | 1:400 | — | — |
*Antigen retrieval was performed using 10 mm Tris, pH 9, and 1 mm EDTA in a steam cooker for 20 min.
aGift from Martin Eilers, University of Würzburg.
bDr. Christian Sartori GmbH & Co. KG.