Table 2.
List of qRT-PCR primers used
| Gene ID | Primer sequence (5′-3′) | Exon junction | Amplicon |
|---|---|---|---|
| Adgre1 | fw: GGAGGACTTCTCCAAGCC | Exon 14/15 | 70 bp |
| rv: AGGCCTCTCAGACTTCTG | |||
| Cdc20 | fw: GCCCACCAAAAAGGAGCATC | Exon 2/3 | 100 bp |
| rv: ATTCTGAGGTTTGCCGCTGA | |||
| Cdk1 | fw: ACACACGAGGTAGTGACGCT | Exon 5/6 | 83 bp |
| rv: CAGATGTCAACCGGAGTGGA | |||
| Cdk4 | fw: GAGCGTAAGATCCCCTGCTTC | Exon 1/2 | 94 bp |
| rv: GGCTTTGTACACCGTCCCAT | |||
| Cdk6 | fw: ACCCACAGAAACCATAAAGGATA | Exon 3/4 | 94 bp |
| rv: GCGGTTTCAGATCACGATGC | |||
| Cdkn1a | fw: TCCACAGCGATATCCAGACA | Exon 1/2 | 90 bp |
| rv: GGCACACTTTGCTCCTGTG | |||
| Ccl2 | fw: CATCCACGTGTTGGCTCA | Exon 1/2 | 76 bp |
| rv: GATCATCTTGCTGGTGAATGAGT | |||
| Ccna2 | fw: CAAGACTCGACGGGTTGCTC | Exon 2/3 | 74 bp |
| rv: TCCATGAAGGACCAGCAGTG | |||
| Ccnb2 | fw: CCGACGGTGTCCAGTGATTT | Exon 1/2 | 76 bp |
| rv: GCCGGATAGTCACATGGCTC | |||
| Ccnd1 | fw: CAAGTGTGACCCGGACTGC | Exon 3/4 | 62 bp |
| rv: TTGACTCCAGAAGGGCTTCAA | |||
| Ccnd2 | fw: CTGCGGAAAAGCTGTGCATT | Exon 2/3 | 72 bp |
| rv: CTGCGGAAAAGCTGTGCATT | |||
| Ccne1 | fw: GACACAGCTTCGGGTCTGAG | Exon 1/2 | 71 bp |
| rv: TGGTCCGTCGAGTCTCTCTC | |||
| Cenpf | fw: ACAGCACAGTATGACCAGGC | Exon 7/8 | 105 bp |
| rv: AGCACTCTCTGCGTTCTGTC | |||
| Ckap2 | fw: GAGGAATCCCTTGCCGGTG | Exon 1/2 | 84 bp |
| rv: TGTTTTCTTTGTTCTCGGAAGGC | |||
| E2F1 | fw: GCAGATGGTGGGGCTGATATT | Exon 1/2 | 97 bp |
| rv: CCGTTTCACCTCCAACAAGC | |||
| Fam64 | fw: CCCCTGTTCCTCCACAGAAC | Exon 3/4 | 110 bp |
| rv: TGTCCAGCCTTTGAGACAGC | |||
| Foxm1 | fw: CTGTGAGGGTCAAAGCTTGC | Exon 1/2 | 94 bp |
| rv: GTCTTTTGAGAATCAGTGGCCG | |||
| Iqgap3 | fw: CTCGGACAGCCTATGAGCG | Exon 1/2 | 85 bp |
| rv: AGCCGGCAGAGGTACTGATA | |||
| Itgam | fw: CCTTGTGGGTAAGCATAGCTAAAT | — | 75 bp |
| rv: CAGCTCACACAAACAAGGAAATA | |||
| Jun | fw: CCAGAAGATGGTGTGGTGTTT | — | 63 bp |
| rv: CTGACCCTCTCCCCTTGC | |||
| Kdm8 | fw: AAAGTTGTCGCAGTCCTCCA | Exon 2/3 | 100 bp |
| rv: CGTCACACTTTGCCTTCTTGG | |||
| Kif20a | fw: GTCCGAAAGGACCTGTTGTC | Exon 2/3 | 72 bp |
| rv: CTCCAGTAGAGCCTGCTTGT | |||
| Kif2c | fw: AGTCCATGGAGTCGCTTCAC | Exon 1/2 | 82 bp |
| rv: GCGGGGTGAATTAAGCCATT | |||
| Kif22 | fw: GTGAGGTGGAGGACTTGGAAC | Exon 13/14 | 80 bp |
| rv: GCTCAAGAGATTCGCCTTCA | |||
| Kif4 | fw: GGCCTCCACAGCTATGAACT | Exon 5/6 | 112 bp |
| rv: ATGCAGCTTGGAACGAAAGC | |||
| Mbp | fw: TGGCCACAGCAAGTACCAT | — | 75 bp |
| rv: AGTCAAGGATGCCCGTGT | |||
| Mki67 | fw: GCTGTCCTCAAGACAATCATCA | Exon 7/8 | 71 bp |
| rv: GGCGTTATCCCAGGAGACT | |||
| Mpz | fw: CCCTGGCCATTGTGGTTTAC | — | 92 bp |
| rv: CCATTCACTGGACCAGAAGGAG | |||
| Nsd1 | fw: ATTCTCGGGGCCGTCCAATA | Exon 1/2 | 95 bp |
| rv: CTGTGCCTGCATCAACCTCA | |||
| Nsd2 | fw: GAGTACGTGTGTCAGCTGTG | Exon 9/10 | 101 bp |
| rv: CTTCGGGAAAGTCCAAGGCA | |||
| Nsd3 | fw: CGGTGTCTCTCCTGTGATCG | Exon 1/2 | 73 bp |
| rv: TCTCTCATCGAGCCTTCAGT | |||
| Nrg1 (I) | fw: GGGAAGGGCAAGAAGAAGG | — | 145 bp |
| rv: TTTCACACCGAAGCACGAGC | |||
| Plk1 | fw: GGTTTTCAATCGCTCCCAGC | Exon 5/6 | 82 bp |
| rv: AGGGGGTTCTCCACACCTTT | |||
| Prc1 | fw: CCAAACAGGAGAGGCAACTG | Exon 10/11 | 109 bp |
| rv: GACTTCTTGGGCGTCTGTCC | |||
| Prx | fw: AGGAGCTCTGGAGGTGTCTGG | Exon 1/3 | 170 bp |
| rv: TCTTGAGTGATGGCCTTTTC | |||
| Psrc1 | fw: TCCCGGGAACTGGGAGATT | Exon 1/2 | 65 bp |
| rv: GGCTTAGACCTCGCTTCACA | |||
| Setd | fw: CCCGACCCCTGAAGAAGAAG | Exon 1/2 | 87 bp |
| rv: CCCGACCCCTGAAGAAGAAG | |||
| Setmar | fw: GGCCTAAACCTTTCCAGCTACT | Exon 1/2 | 107 bp |
| rv: CCGGGTGCTGTCTTACTCAG | |||
| Sox2 | fw: TCCAAAAACTAATCACAACAATCG | — | 73 bp |
| rv: GAAGTGCAATTGGGATGAAAA | |||
| Top2a | fw: CGAGAAGTGAAGGTTGCCCA | Exon 19/20 | 113 bp |
| rv: CCCACAAAATTCTGCGCCAA | |||
| Tpx2 | fw: ATAGGCGAGCCTTTTCAGGG | Exon 4/5 | 82 bp |
| rv: CAACTGCCTTCAACGGTGTG | |||
| Zbtb17 | fw: AGGCACACTGTCTGAGAAGAGA | Exon 2/3 | 96 bp |
| rv: TGGTTCAGCTGCTCCAAGA | |||
| Znhit3 | fw: GAAGCCGAAATACCGTTGCC | Exon 1/2 | 83 bp |
| rv: AGCTGCACTGCTCTTTGTGC |