Table 4.
Identification of Kdm8 and Znhit3 as direct Miz1 target genes
| Gene | Primer sequence | INR motif | MIZ1 motif | Amplicon | Fold enrichment |
|---|---|---|---|---|---|
| Tnfaip2 | AGGCGTTGAGATGGAAGAGG | −145 | −144 | −172 to −114 | 1.000 ± 0.046 |
| CGTCACACAGTCAGCACCTA | |||||
| Tnfaip2 | GAACTCGTGGCTCCCTGT | −1 | −144 | −34 to 36 | 0.995 ± 0.045 |
| GGTCACTTTGCTGTTCCCAA | |||||
| Crlf1 | CGCCTTGTCAATTTCGGCTG | −26 | — | −33 to 54 | 1.375 ± 0.365 |
| CAGATTGGGGCTCGGGTC | |||||
| Psrc1 | GCAAGGAAACCCAGTCTCAGA | −333 | — | −333 to −255 | 1.105 ± 0.045 |
| GGGGAGAACTGTCGAACCAA | |||||
| Klk8 | TCACAAGTGGGCCAGAACTC | −168 | 318 | 249 to 338 | 1.045 ± 0.095 |
| GCAGAGCTCTAGATCCCCGA | −141/155 | ||||
| Hmmr | CGACGATCAGGAGCAGCC | −131 | — | −166 to −106 | 1.345 ± 0.065 |
| CACTAGATTCCTACCGCCGC | |||||
| Iqgap3 | TCGGACAGCCTGTGAGTG | 280 | 150 | 111 to 180 | 0.805 ± 0,155 |
| GAAAACCAAGCCCCGCCA | |||||
| Ckap2 | CAGCATTTGACAGGTGCAGT | −171 | −239 | −260 to −200 | 1.045 ± 0.025 |
| TCTTCAGACACAGCCCAGAG | |||||
| Top2a | GTCTCTCAGAGCACATGGACC | −222 | — | −254 to −161 | 1.075 ± 0.125 |
| GGCGGGAGAATCTTCAAAATGG | |||||
| Kif20a | CACGCCTGAGAATTGGGATG | −130 | — | −158 to −77 | 1.150 ± 0.010 |
| GGACAGGCAGTGAGTATCGG | |||||
| Lipo1 | ACCTGCATTCTTCACAACTTTGG | −219 | — | −259 to −188 | 0.950 ± 0.110 |
| ACATTGGCTCCTCAGTGACC | |||||
| Cenpf | GCACAGACCTCACCTTACCC | −254 | — | −316 to −244 | 0.930 ± 0.090 |
| GCGGGTCTGAATGAGGGTTA | |||||
| Tpx2 | CTCTCTGATTGGTGCGTTCG | 50 | 58 | 15 to 87 | 1.595 ± 0.375 |
| CCGCGTCAGGACTCAACT | |||||
| Pbk | GCTATGTCTTTCTAGCCATCGC | −71 | — | −122 to −29 | 0.935 ± 0.015 |
| TTCGCACAGTCCTCCCAG | |||||
| Mki67 | CTTCCCCAATCCTCTGTCGC | −188 | — | −193 to −102 | 1.130 ± 0.090 |
| AACGGCGGTTCAAATTCTGG | |||||
| Prc1 | TAGGCGGTCTCTGTATCCCA | −220/−212 | −108 | −126 to −27 | 1.183 ± 0.494 |
| GACAATTGGTTACCGCGTCC | |||||
| Kif4 | TGTCACCACTTCCAACCTAGG | −89 | — | −94 to −1 | 1.095 ± 0.105 |
| ATGGGATAGTTAAGAGTTGGCAC | |||||
| Kif22 | GAAGGAAAAGTCGGGCGAAA | −233 | — | −265 to 206 | 1.130 ± 0.086 |
| TGAGGTCCCTGCACTTTCAG | |||||
| Fam64 | GAGGACCGATAAGAAGGCCC | −493 | — | −543 to −459 | 1.125 ± 0.095 |
| TCTAACTGGCCACACTCAGC | |||||
| Kif2c | ACTGGTGACGTTGTAGGGAG | −235/−75 | 61 | 10 to 98 | 1.295 ± 0.275 |
| CGACTCTGCTAATACTGCGC | |||||
| Eda2r | CCTCCCTAGACCCAGCTTCA | −179/−139 | — | −184 to −99 | 0.940 ± 0.070 |
| TGAGTCTGGCGGGTAAACAC | −108/−64 | ||||
| Nusap1 | AAAACAAACCCCTCCCTCCC | −189/160 | — | −234 to −141 | 1.070 ± 0.240 |
| TGAGTGAGGCGAGGGTAGTT | |||||
| Nrg1(I) | GGACTTCCCCAAAACTTGTGG | — | −3 | −63 to 27 | 1.309 ± 0.409 |
| CTCGCATCTCCCGGCAG | |||||
| Kdm8 | CGATCAGCCGAGTCAGAAGT | 148 | 36 | −14 to 48 | 158.5 ± 23.69 |
| CTTCGGCTGGCTTTATCACC | |||||
| Znhit3 | CGACACTCTACAGGTGAGGC | −370 | −108 | −107 to −65 | 119.6 ± 13.76 |
| TGGCAGTCAGAAGCAATGGA |
The table documents the primers used for the ChIP analysis using the anti-Miz1 antibody H190, and the fold enrichment obtained. INR, Initiation region; Miz1 motif, Position of the Miz1 consensus binding region, if one were found by the MAST algorithm; Amplicon, amplified region. Numbers indicate the position in relation to the TSS.