Table 1.
Primary and secondary antibodies
Species | Concentration | Source (catalog No.) | |
---|---|---|---|
Primary antibodies | |||
Anti-BrdU | Rat | 1:500 | ABD Serotech (ABD0030) |
Anti-BrdU | Rat | 1:500 | Abcam Ab6326 |
Anti-calretinin | Rabbit | 1:1000 to 1:2000 | SWANT (7697) |
Anti-calretinin | Mouse | 1:2000 | SWANT (6B3) |
Anti-calbindin | Mouse | 1:2000 | SWANT (D28K) |
Anti-cFos | Goat | 1:50 | Santa Cruz Biotechnology (Sc-52-G) |
Anti-TH | Mouse | 1:400 | Millipore (MAB318) |
Anti-GFP | Chicken | 1:1000 | JAVES (GFP-1020) |
Anti-doublecortin | Goat | 1:500 | Santa Cruz Biotechnology (Sc-8066) |
Anti-RFP | Rabbit | 1:1500 | MBL (PM005) |
Secondary antibodies | |||
Anti-rat AlexaFluor 555 | Donkey | 1:1000 | ThermoFisher Scientific (A-21434) |
Anti-rat AlexaFluor 546 | Goat | 1:200 | ThermoFisher Scientific (A-11081) |
Anti-mouse AlexaFluor 555 | Donkey | 1:1000 | ThermoFisher Scientific (A-31570) |
Anti-mouse AlexaFluor 647 | Donkey | 1:1000 | ThermoFisher Scientific (A-31571) |
Anti-rabbit AlexaFluor 555 | Donkey | 1:1000 | ThermoFisher Scientific (A-31572) |
Anti-rabbit AlexaFluor 647 | Donkey | 1:1000 | ThermoFisher Scientific (A-31573) |
Anti-rabbit AlexaFluor 633 | Goat | 1:200 | ThermoFisher Scientific (A-21070) |
Anti-goat AlexaFluor 555 | Donkey | 1:1000 | ThermoFisher Scientific (A-21432) |
Anti-chicken biotinylated | Donkey | 1:1000 | The Jackson Laboratory (703-065-155) |
Streptavidin-DTAF | — | 1:250 | The Jackson Laboratory (016-010-084) |