Table 1.
Relaxation times (T1) in a mixture of 2.5 mM PCr, 3.5 mM ATP, and 1.0 mM Pi while varying type and concentration of modifiers in the matrix
| Concentration | PCr | γ-ATP | Pi | Concentration | PCr | ɣ-ATP | Pi |
|---|---|---|---|---|---|---|---|
| T1 (ms) | T1 (ms) | T1 (ms) | T1 (ms) | T1 (ms) | T1 (ms) | ||
| Gd (μM) | Mg (mM) | ||||||
| 0 | 5812 | 5608 | 7269 | 0 | 5812 | 5608 | 7269 |
| 0.1 | 4706 | 1780 | 7300 | 1 | 5365 | 3393 | 7600 |
| 0.2 | 4600 | 1163 | 6820 | 1.5 | 3636 | 7665 | |
| 1 | — | 263 | — | 2 | 5431 | 3566 | — |
| 1.5 | 4200 | — | 6200 | 2.5 | 5401 | 3358 | 6666 |
| 8 | 3501 | — | 4218 | 3.5 | 5693 | 3250 | 6848 |
| 14 | 2093 | — | 2722 | 6 | 4948 | 2499 | 6074 |
| 25 | 1486 | — | 1994 | 10 | 5213 | 1795 | 6300 |
| Agarose (%) | K (mM) | ||||||
| 0 | 5812 | 5608 | 7269 | 140 | 5061 | 3793 | 7285 |
| 0.07 | 5170 | 1468 | 7253 | ||||
| 0.2 | 5600 | 765 | 6700 | K + Mg (mM) | |||
| 0.4 | 5091 | 325 | 4643 | 140 + 1.5 | 5016 | 3044 | 6688 |
| 0.6 | 4939 | — | 4161 |
At each measurement 1.5 L of solution adjusted to pH 7.4 was submitted to a 31P spectroscopy saturation study at 13 different repetition times. T1 were determined using Equation 1.