Table 1.
Detailed information on the primary antibodies used in this study
| Antibody (anti-) | Manufacturer, catalog # | Marker of | Working dilution |
|---|---|---|---|
| Actin | Sigma-Aldrich, A5441 | Housekeeping protein | 1:6000 |
| Atg5 | Cell Signaling Technology, 12994 | Autophagy initiation | 1:1000 |
| Beclin1 | Cell Signaling Technology, 3495 | Autophagosome induction | 1:1000 |
| CB | Swant, 300 | Calbindin interneurons | 1:2000 |
| CMV | S. Jonjic gift | IE1 protein of the murine virus | 1:1000 |
| CR | Swant, 7699/3H | Caretinin interneurons | 1:2000 |
| Active casp3 | Cell Signaling Technology, asp175 | Apoptotic cells | 1:300 |
| CD68 | Serotec, MCA1957GA | Activated microglia | 0.5 μg/ml |
| Iba1 | Wako Chemicals, 016-20001 | Microglia | 1:500 |
| Ki67 | Abcam, 15580 | Proliferating cells | 1:1000 |
| Lamp1 | Abcam, 24170 | Lysosomes (autophagy) | 1:1000 |
| LC3 | Sigma-Aldrich, L7543 | Autophagic flux | 1:2000 |
| OMP | Wako, 544-10001 | Olfactory sensory neurons | 1:1000 |
| P62 | Abnova, H00008878-M01 | Autophagic flux | 1:1000 |
| pAMPK1 | Cell Signaling Technology, 2531 | Metabolic signalling | 1:1000 |
| pmTOR | Cell Signaling Technology, 2971 | Immune signalling | 1:1000 |
| TH | ImmunoStar, 22941 | Dopaminergic neurons | 1:4000 |