Table 2.
Effect of TCB-2 on electrophysiological features of recorded interneurons within MDH lamina IIi
| Cell type | Condition | Membrane properties |
Action potential characteristics |
|||||
|---|---|---|---|---|---|---|---|---|
| Rm (MΩ) | Cm (pF) | RMP (mV) | Rh (pA) | Firing threshold (mV) | Amplitude (mV) | Duration (ms) | ||
| PKCγ+ | aCSF (4) | 338.8 ± 102.5 | 69.5 ± 16.6 | −58.4 ± 2.2 | 86.3 ± 21.5 | −39.0 ± 6.0 | 38.7 ± 5.5 | 2.0 ± 0.2 |
| TCB-2 10 μm (4) | 362.0 ± 102.2 | 82.3 ± 28.3 | −57.9 ± 2.6 | 117.5 ± 47.7 | −30.3 ± 7.8 | 38.2 ± 9.6 | 2.0 ± 0.4 | |
| PKCγ− | aCSF (5) | 318.8 ± 49.9 | 45.8 ± 9.8 | −63.0 ± 3.6 | 116.0 ± 48.0 | −36.4 ± 5.5 | 48.6 ± 7.3 | 2.2 ± 0.2 |
| TCB-2 10 μm (5) | 310.8 ± 65.3 | 47.7 ± 9.6 | −63.1 ± 1.5 | 114.0 ± 37.5 | −39.4 ± 2.3 | 48.4 ± 4.1 | 2.0 ± 0.2 | |
Data represent mean ± SEM of electrophysiological parameters in ex vivo (n) recorded (patch-clamp recordings) PKCγ+ and PKCγ− interneurons before (aCSF) and after bath application of TCB-2 (10 μm, 5-HT2AR agonist). Cm, Membrane capacitance.