Table 4.
Morphological features of PKCγ− interneurons within MDH lamina IIi
| Vehicle (8) | CFA (12) | CFA + 4F4PP 100 nm (15) | TCB-2 10 μm (11) | 5HT 10 μm (9) | |
|---|---|---|---|---|---|
| Soma volume (μm3) | 314.0 ± 38.3 | 273.3 ± 63.8 | 133.6 ± 14.8 | 336.2 ± 70.9 | 383.5 ± 75.8 |
| Total field area (103 μm2) | 9.7 ± 2.7 | 9.9 ± 1.0 | 6.0 ± 0.7 | 7.8 ± 1.6 | 9.5 ± 2.1 |
| Branch density (branch number/103 μm3) | 4.5 ± 1.2 | 3.3 ± 0.4 | 5.2 ± 0.7 | 5.4 ± 0.9 | 3.9 ± 0.8 |
| Fractal dimension | 1.21 ± 0.03 | 1.20 ± 0.03 | 1.13 ± 0.01 | 1.14 ± 0.02 | 1.17 ± 0.03 |
| Number of branches | |||||
| Total | 28.1 ± 2.9 | 28.8 ± 2.9 | 26.5 ± 3.2 | 31.7 ± 4.5 | 27.8 ± 5.7 |
| Primary branches | 8.0 ± 0.8 | 9.7 ± 1.2 | 8.3 ± 0.5 | 9.3 ± 0.8 | 7.3 ± 1.2 |
| Secondary branches | 9.1 ± 1.6 | 9.8 ± 1.2 | 8.8 ± 0.9 | 11.5 ± 1.6 | 8.2 ± 2.0 |
| Tertiary branches | 7.3 ± 1.1 | 5.3 ± 1.0 | 5.5 ± 1.1 | 7.1 ± 1.1 | 6.1 ± 2.2 |
| Quaternary branches | 3.6 ± 1.4 | 4.0 ± 1.2 | 3.9 ± 1.6 | 3.8 ± 2.2 | 6.1 ± 1.8 |
| Length of branches | |||||
| Total mean length | 22.1 ± 3.1 | 19.1 ± 1.7 | 15.3 ± 1.2 | 17.4 ± 1.6 | 25.9 ± 3.2 |
| Primary branches | 22.7 ± 3.9 | 17.3 ± 2.1 | 14.9 ± 0.8 | 16.2 ± 1.5 | 23.6 ± 2.4 |
| Secondary branches | 17.4 ± 3.2 | 19.2 ± 1.4 | 17.4 ± 1.6 | 19.6 ± 2.6 | 27.8 ± 5.6 |
| Tertiary branches | 22.2 ± 2.6 | 22.6 ± 4.2 | 13.9 ± 1.8 | 18.1 ± 1.6 | 26.2 ± 4.8 |
| Quaternary branches | 29.1 ± 6.6 | 15.2 ± 1.3 | 13.2 ± 1.8 | 13.2 ± 2.6 | 27.2 ± 7.7 |
| Number of nodes | 8.5 ± 1.3 | 9.7 ± 1.3 | 7.7 ± 1.3 | 9.5 ± 1.6 | 9.2 ± 2.3 |
| Number of end points | 19.4 ± 1.8 | 21.0 ± 2.0 | 18.0 ± 1.7 | 22.3 ± 3.0 | 17.2 ± 2.4 |
Data represent mean ± SEM of morphological and structural characteristics of (n) 3D-reconstructed neuritic arbors of neurobiotin-labeled PKCγ− interneurons recorded in ex vivo MDH slices from vehicle, CFA (subcutaneous injection into the vibrissa pad: 2.5 mg/kg), and after bath application of TCB-2 (10 μm, 5-HT2AR agonist) or 5-HT (10 μm). 4F4PP (100 nm, 5-HT2AR antagonist) was administered intracisternally 30 min before CFA. Neuritic 3D reconstructions were performed from parasagittal slices (350 μm thick).