Skip to main content
. Author manuscript; available in PMC: 2019 Jun 27.
Published in final edited form as: Anal Chem. 2019 Mar 15;91(9):5794–5801. doi: 10.1021/acs.analchem.9b00024

Figure 1.

Figure 1.

BASIL quantitative strategy. (a) The small amount of study samples and the much larger amount of boosting sample were individually labeled with different TMT tags. (b) The differentially labeled peptides appear as a single peak (identical m/z at MS1 level), which represents the sum of intensities from both the study and boosting samples. (c) Sequence information can be obtained after MS/MS fragmentation of the peptide backbone and quantification information on the study samples can be obtained from the intensities of the individual TMT reporter ions.