Table 1.
Strains and vectors used in the study.
| Strain | Description in brief | References |
|---|---|---|
| P-1 | Wild-type strain of U. virens | Zheng et al., 2017 |
| DHOX-17 | UvHOX2 deletion mutant | This study |
| DHOX-21 | UvHOX2 deletion mutant | This study |
| DHOX-51 | UvHOX2 deletion mutant | This study |
| DHOX-61 | UvHOX2 deletion mutant | This study |
| Trans-3 | UF-HYG+-RF ectopic insertion mutant | This study |
| HOX-GFP-2 | HOX-GFP fusion expression mutant | This study |
| Vector | ||
| pmCherry-hph | Harboring mcherry expression- and hygromycin resistant cassettes | Stock of our lab |
| pCambia-1300 | Binary vector for agrobacterium tumefaciens transformation (ATMT) | Cambia |
| pBHt2 | Binary vector for ATMT of filamentous fungi | Mullins et al., 2001 |
| pKHt | Binary vector containing eGFP expression cassette | Mullins et al., 2001 |
| pCN3EXPS | Binary vector for over-express of genes in filamentous fungi | Stock of our lab |
| AGL-1 | Agrobacterium tumefaciens strain | Presented by Dr. Lijun Ma |
| pCas9-tRp-gRNA | Containing CRISPR/Cas9 cassette and gRNA cassettes controlled by Gln-tRNA | Liang et al., 2018 |
| pCas9-tRPD | Two Aar I restriction sites were introduced in the flanking regions of CRISPR/Cas9 and gRNA cassettes | This study |
| pCas9-tRPDI | pCas9-tRPD inserted with gRNA spacers SP1S-SP1AS | This study |
| pCas9-tRPDII | pCas9-tRPD inserted with gRNA spacers SP2S-SP2AS | This study |
| Pcccd | Binary vector containing a ccdB toxic cassette in T-DNA 1 region | This study |
| pCccd-dT | Binary vector containing two T-DNA regions | This study |
| pCccd-dTN3 | A geneticin 418-resistant cassette added into T-DNA 1 region | This study |
| pdTN3-HX2-Cas9I | For deletion of UvHOx2 | This study |
| pdTN3-HX2-Cas9II | For deletion of UvHOx2 | This study |