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. 2019 Jun 20;10:1413. doi: 10.3389/fmicb.2019.01413

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Copyright © 2019 Anantharajah, Tossens, Olive, Kabamba-Mukadi, Rodriguez-Villalobos and Verroken.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Scheme illustrating the MBT STAR^®-Carba IVD workflow from colonies and from spiked blood cultures. One to five colonies from overnight cultures or the pellet obtained from positive blood cultures by the MBT Sepsityper kit were mixed with the reconstituted MBT STAR^®-Carba Antibiotic Reagent containing imipenem. After incubation and centrifugation, cell-free supernatant is spotted onto a MALDI target plate and overlaid with matrix. Spectra are then acquired using the MALDI Biotyper^®smart system and analyzed by the MBT STAR^®-BL IVD module.