Table 1.

Performances of the MBT STAR^®-Carba IVD Assay for the detection of carbapenem hydrolysis in carbapenem resistant organisms.

	Carbapenemase	No. of isolates	Incubation time (min)	Assay results
				Hydrolysed	Non-hydrolysed	Sensitivity (95% CI*)	Specificity (95% CI*)
A. Assay performed on bacterial colonies
Enterobacteriaceae (n = 89)						100% (95.3–100.0)	100% (74.1–100.0)
K. pneumoniae (n = 49), E. coli	OXA-48-like	41	30	41	–
(n = 22), K. oxytoca (n = 5)	NDM	27	30	27	–
E. cloacae (n = 6), C. freundii	KPC	6	30	6	–
(n = 4), E. aerogenes (n = 2),	VIM	4	30	4	–
P. stuartii (n = 1)	Negative	11	30	–	11
Pseudomonas spp. (n = 29)						100% (82.4–100.0)	100% (74.1–100.0)
P. aeruginosa (n = 28),	VIM	17	30	17	–
P. chlororaphis (n = 1)	GES (ESBL)	1	30	–	1
	IMP	1	30	1	–
	Negative	10	30	–	10
A. baumannii complex (n = 12)						100% (75.7–100.0)	NA#
	OXA-23	8	60	8	–
	OXA-24/-40	2	60	2	–
	OXA-58	2	60	2	–
B. Assay performed on positive blood cultures
Enterobacteriaceae (n = 26)						100% (85.7–100.0)	66.7% (20.8–93.8)
K. pneumoniae (n = 16),	OXA-48-like	12	60	12	–
E. coli (n = 5), E. cloacae	NDM	5	60	5	–
(n = 2), C. freundii (n = 2),	KPC	4	60	4	–
E. aerogenes (n = 1)	VIM	2	60	2	–
	Negative	3	60	1	2
Pseudomonas spp. (n = 7)						100% (51.0–100.0)	100% (43.8–100.0)
P. aeruginosa (n = 6),	VIM	3	60	3	–
P. chlororaphis (n = 1)	IMP	1	60	1	–
	Negative	3	60	–	3
A. baumannii complex (n = 12)						25%/58.3% (8.3–53.2)/(31.9–80.7)	NA#
	OXA-23	8	60/120	2/4	6/4
	OXA-24/-40	2	60/120	–/2	2/–
	OXA-58	2	60/120	1/1	1/1

*95% CI: 95% confidence interval. ^#NA: non-applicable.