Skip to main content
. 2019 Jun 20;12:153. doi: 10.3389/fnmol.2019.00153

FIGURE 5.

FIGURE 5

Effects of IL-1β (10 ng) on the early increases in the expression of spinal P450c17 and astrocyte activation in IL-1 receptor antagonist (IL-1ra, 20 ng) treated CCI mice. (A) Results of Western blot analysis showed that the protein expression of P450c17 in the ipsilateral spinal cord dorsal horn was increased at day 1 post-CCI surgery by administration of IL-1ra, and this increase was blocked by the co-administration of IL-1β (10 ng) with IL-1ra. (B) Results of Western blot analysis showed that the protein expression of GFAP in the ipsilateral spinal cord dorsal horn was increased at day 1 post-CCI surgery by administration of IL-1ra, and this increase was blocked by co-administration of IL-1β (10 ng) with IL-1ra. (C,D) The expression of P450c17 (C) and GFAP (D) in the contralateral dorsal horn did not show any change after drug administration. The spinal cord dorsal horn was sampled at day 1 post-surgery. n = 6 mice/group. ∗P < 0.05 vs. vehicle-treated group; #P < 0.05 vs. IL-1ra-treated group.