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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Stem Cells. 2019 Jan 24;37(4):453–462. doi: 10.1002/stem.2969

Figure 4. Angiogenic Effects of in vitro and in vivo Modulation of CHD7 Expression.

Figure 4.

(A) Representative images of tube formation after D456 GBM PDX or neural progenitor cells expressing CHD7 shRNA or NT control were co-cultured with human brain microvascular endothelial cells. (B) Quantification of branch length and number of segments in the tube formation assay. (C) Representative images at 10x magnification of sections obtained from orthotopic xenografts formed by intracranial injection of D456 cells expressing shRNA specific for CHD7. Immunohistochemistry shown represents CHD7 expression and blood vessel formation by CD31 staining. Abnormal vasculature and thrombosis marked by arrowheads. (D) Quantification of CD31 staining as a percentage of total slide micrograph area from three tumor sections. *, p<0.05 with ANOVA.