. 2019 Jun 20;10:1416. doi: 10.3389/fmicb.2019.01416

TABLE 1.

Detailed information on the primers used for screening for the equine viral pathogens associated with fever.

Virus	Viral	Targeting	Primer sequence (5′→3′)	Annealing	PCR
	genome type	viral gene		temperature	product
	(RNA/DNA)				length
African horse	RNA	Segment 7	ASFV-F: GTTAAAATTCGGTTAGGATG;	55°C	1179 bp
sickness virus			ASFV-R: GTAAGTGTATTCGGTATTG
Equine arteritis virus	RNA	ORF1b	CE^a: TGGTAGGTGCTTCATTGGCT	42°C^a,b	186 bp
			DE^a: GCGGCACAAGAACACTTCTG
			CI^b: CCTGAGACACTGAGTCGCGT
			DI^b: CCTGATGCCACATGGAATGA
Equine infectious	RNA	gag	P1^a:GTAATTGGGCGCTAAGTCTAG	56°C^a,b	211 bp
anemia virus			P2^a: CCTCTAATAAATCTTGCTGTC
			P4^b:TGGGTGAATACCATACAGACA
			P5^b: CCAGTGGAGCATTCGGTAA
Equid	DNA	gH	F^a:AAGAGGAGCACGTGTTGGAT	60°C^a,b	287 bp
herpesviruses 1			R^b:TTGAAGGACGAATAGGACGC
			RN^a,b:AGTAGGTCAGGCCGATGCTT
Equid	DNA	gB	F^a:CTGCTGTCATTATGCAGGGA	60°C^a,b	323 bp
herpesviruses 4			R^b:CGTCTTCTCGAAGACGGGTA
			RN^a,b:CGCTAGTGTCATCATCGTCG
Japanese	RNA	Cap	JEV19F^a:ACTTCTTGGCTTAGTATCGT	45°C^a;	227 bp
encephalitis virus			JEV535R^a:GCAATGTCCGTGTTGTT	50°C^b
			JEV135F^b:ATCAATATGCTGAAACGCGG
			JEV361R^b:CCAAGTTCTCGTTTGAAACT
Getah virus	RNA	Cap	SagW8:CCATGGTTATTCCTGAGCTGCAAA	50°C	590 bp
			SagW9:CCACACGTCCTTTGTTGTCGAAGA
		nsP1	M2W-S:CAGAGCATTTTCGCATCTGGCTA	50°C	434 bp
			M3W-S:ACATGAATGGAGTGGTGTCGAATCCAATCC
Equine hepacivirus	RNA	NS3	NS3O-F^a ATHTGTGATGARTGCCAYAGYAC	55°C	127bp
			NS3O-R^a TAGTAGGTBACAGCRTTAGCYCC
			NS3I-F^b TCYAARGGTGTDAAGCTTGTTGT
			NS3I-R^b TGRCARAAGYTAAGRTGYCTYCC
Equine parvovirus	DNA	VP	ak5^a GTCGCTGCATTCTGAGTCC	55°C	587bp
			ak^6a TGGGATTATACTGTCTACGGGT
			ak7^b CTGCATTCTGAGTCCGTGGCC
			ak8^b CTGTCTACGGGTATCCCATACGTA

^aPrimer/annealing temperature in the first round of nested PCR. ^bPrimer/annealing temperature in the second round of nested PCR.