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. 2019 Jun 20;10:774. doi: 10.3389/fpls.2019.00774

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Copyright © 2019 Zelkowski, Zelkowska, Conrad, Hesse, Lermontova, Marzec, Meister, Houben and Schubert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Localization of NSE4A during the meiosis of A. thaliana (A,B) and the closely related species B. rapa (C). (A) Line-like NSE4A-GFP signals are detectable in an unfixed meiocyte at pachytene of a transgenic pnse4A::gNse4A::GFP A. thaliana plant. (B) Dynamics and localization of NSE4A-GFP signals during meiosis of pnse4A::gNse4A::GFP transgenic A. thaliana plants, detected by anti-GFP. The NSE4A-GFP signals are detectable in G2, leptotene, zygotene, and pachytene cells. The signals are weak or not visible in condensed metaphase I and anaphase I chromosomes, respectively, but are recovered in prophase II, tetrads and young pollen. (C) Anti-AtNSE4A labels the synaptonemal complex of B. rapa and colocalizes to ZYP1 during pachytene. Gray color indicates chromatin counterstained with DAPI. Bars = 10 μm.