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. 2019 Jun 20;10:774. doi: 10.3389/fpls.2019.00774

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Copyright © 2019 Zelkowski, Zelkowska, Conrad, Hesse, Lermontova, Marzec, Meister, Houben and Schubert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The distribution of NSE4A in differentiated somatic flower bud and 8C leaf interphase nuclei analyzed by 3D-SIM. In agreement, both NSE4A antibodies (anti-NSE4A) and NSE4A-EYFP signals detected by anti-GFP antibodies indicate that NSE4A is distributed within euchromatin, but absent from heterochromatin (DAPI-intense chromocenters, arrows). The NSE4A labeling visible in the merged image of the 8C nucleolus (maximum intensity projection) originates from optical sections outside of the nucleolus.