Table 1.
Characterization of the T-DNA insertion mutants of the A. thaliana Nse4 genes.
| Gene symbol | T-DNA mutant | Zygosity | Habit | Pollen fertility (%) | Silique length (mm) | Seeds per silique | Shriveled seeds per silique | % mitotic cells with bridges/fragments | % meiocytes with bridges/fragments |
||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Metaphase I | Anaphase I | Anaphase II | |||||||||
| Col-wt | – | – | wt | 100 (10790) | 12.8 (28) | 44.5 (1468) | 0.7 | 1.5 (417) | 0 (60) | 1.2 (67) | 0 (23) |
| Nse4A | Salk_057130 | He | Smaller | 98.2 (3808) | 11.3∗∗ (24) | 30.3∗∗ (726) | 2.7∗∗ | 11.9∗∗ (242) | 8.4∗ (59) | 4.2 (47) | 10.0 (21) |
| SAIL_71_A08 | He | ||||||||||
| GK-768H08 | Ho | Smaller | 50.2∗∗ (6396) | 10.0∗∗ (25) | 21.0∗∗ (525) | 7.8∗∗ | 25.7∗∗ (175) | 25.2∗∗ (115) | 40.4∗∗ (114) | 47.4∗∗ (19) | |
| GK-768H08 (complemented) | Ho | wt-like | 102 (6270) | 12.3∗ (25) | 31.8∗∗ (795) | 3.4∗∗ | 2.6 (373) | 5.0∗∗ (121) | 19.1∗∗ (131) | 15.0∗ (20) | |
| Nse4B | SAIL_296_F02 | Ho | wt-like | 97.2 (3770) | 10.8∗∗ (30) | 30.5∗∗ (916) | 1.6 | 2.1 (278) | 0 (59) | 5.9 (85) | 8.3 (12) |
| GK-175D11 | Ho | wt-like | 64.3∗∗ (3206) | 11.3∗∗ (30) | 34.8∗∗ (1080) | 2.9∗∗ | 1.7 (178) | 6.2∗∗ (113) | 17.8∗∗ (106) | 0 (18) | |
| Nse4A/Nse4B | GK-768H08/SAIL_294_F02 | Ho/ho | Smaller | 34.8∗∗ (4529) | 10.2∗∗ (30) | 17.9∗∗ (536) | 5.6∗∗ | 28.6∗∗ (619) | 30.6∗∗ (72) | 65.0∗∗ (172) | 50.0∗∗ (34) |
The numbers of pollen, siliques, seeds, somatic and meiotic cells analyzed are indicated in parentheses. ∗∗p < 0.01; ∗p < 0.05.