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. 2019 Jun 20;10:1382. doi: 10.3389/fimmu.2019.01382

Figure 4.

Figure 4

The functions of L-PDCD5 in the immune responses of lampreys. (A) Q-PCR analysis of the expression patterns of L-PDCD5 in leukocytes at 0, 6, and 12 h after lampreys were challenged with GCRV. All of the data are presented as the means ± SDs based on three independent cDNA samples with three replicates per sample. The asterisks indicate significant differences (n = 3 and *P < 0.05) compared to the control. (B) Immunofluorescence analysis of PDCD5 surface expression in leukocytes at 0, 12, 24, and 48 h after lampreys were challenged with or without V. anguillarum and GCRV treatment were bound to the rabbit anti-rL-PDCD5 antibody and incubated with FITC-conjugated anti-rabbit IgG secondary antibodies followed by FACS analysis. Numbers above bracketed lines indicate percent PDCD5 positive cells mean fluorescence intensity. Percentages indicate the number of PDCD5 positive cells. (C) Western blotting analysis of the L-PDCD5 expression in leukocytes at different periods after the challenges with V. anguillarum and GCRV infection using the rabbit anti-rL-PDCD5 antibody. β-actin was used as a loading control. All experiments were repeated at least three times with similar results.