Figure 7.

The localization of L-PDCD2, L-PDCD5, and L-PDCD10 and cell morphological changes upon apoptosis were observed in response to DNA damage. (A) Confocal microscopy observation H293T cells transfected with L-PDCD2, L-PDCD5, and L-PDCD10 for 24 h and then treated either with or without CDDP (10³ μmol/L) for 6 h. Hoechst 33342 was used to stain the nucleus. (B) L-PDCD5 enhances the growth inhibition of H293T cells. The left graph shows the transfection efficiency of cells under fluorescence microscopy, while the upper right graph shows the statistics. Furthermore, the lower right graph shows the cell viability of L-PDCD5-overexpressing cells at the indicated time points using a CCK-8 assay. The data are presented as the means ± SDs of the results from three independent experiments (^*P < 0.05, ^**P < 0.01, ^#P < 0.05, and ^##P < 0.01). (C) H293T cells were transfected with L-PDCD2, L-PDCD5, and L-PDCD10 for 24 h and then treated either with or without CDDP (10³ μmol/L) for 6 h. Apoptosis was determined through Annexin V conjugate staining using flow cytometry. L-PDCD2 or L-PDCD5 overexpression increased the apoptotic rate in H293T cells that were either treated with or without CDDP (left side). A histogram showing the statistics of the above mentioned results (right side). All experiments were repeated at least three times with similar results (^*P < 0.05, ^**P < 0.01, ^#P < 0.05, and ^##P < 0.01).