Olfactory neuron cilia and sustentacular cell microvilli express the high affinity GLUT3 glucose transporter. A, Immunocytochemistry for GLUT3 without (left) or with (right) preincubation of the primary antibody (Alpha Diagnostics anti-GLUT3 antibody) with its antigenic peptide in rat OSNs, as a control for the specificity of the antibody. The left three panels show a neuron labeled for OMP, GLUT3, and a merge of both; the two right panels show the control. B, Immunocytochemistry (Sigma anti-GLUT3 antibody) for GLUT3 of an isolated rat OSN. C, Isolated toad OSN (Sigma anti-GLUT3 antibody). D, Rat SCs (Sigma anti-GLUT3 antibody). GLUT3-expressing cilia are indicated by arrowheads. C, D, Insets (3×) correspond to the areas denoted in the figures. Nuclei are labeled blue (TOPRO). Scale bars, 10 μm.