Figure 5.

Gene deletion with Slco1c1-Cre targets mainly endothelial cells in small- and medium-sized vessels in the brain. a, Dual labeling (arrowheads) of the Cre reporter protein tdTomato and Cox-2 in a small vessel in the brain. b, Large vessel with smaller-sized branch. Most of the Cox-2-immunoreactive cells (green) in the large vessel do not express tdTomato (arrows), whereas those in the smaller sized branch do (arrowheads). c, Numerous Cox-2-expressing cells (arrows) in a large vessel are shown, but few of those cells also express tdTomato (arrowheads). All micrographs are from immune-challenged mice. Note that endothelial cells in capillaries express tdTomato (small arrows) but not Cox-2. Scale bar, 20 μm.