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. 2017 Jun 21;37(25):5978–5995. doi: 10.1523/JNEUROSCI.3148-16.2017

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Copyright © 2017 the authors 0270-6474/17/375978-18$15.00/0

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Figure 3. — JM1 of TrkB is both necessary and sufficient for the association between TrkB and UCH-L1. A, Schematic presentation of TrkB-FL, TrkB-T1, JM1, JM0, and ΔJM1 used in this study. B, Co-IP was performed in HEK293 cell lysates expressing Myc-UCH-L1 and Flag-TrkB FL or other TrkB mutants: JM1, JM0, or ΔJM1. Cell lysates were immunoprecipitated (IP) using anti-Flag antibodies. C, Schematic presentation of IL-2R and IL-2R JM1 used in this study. D, Co-IP was performed in HEK293 cell lysates expressing Myc-UCH-L1 and Flag-TrkB FL or IL-2R or IL-2R JM1. Cell lysates were immunoprecipitated using anti-Flag antibodies.